Evaluation of colorimetric loop-mediated isothermal amplification assay for visual detection of Streptococcus agalactiae and Streptococcus iniae in tilapia
نویسندگان
چکیده
منابع مشابه
rapid detection of streptococcus agalactiae infection using a loop-mediated isothermal amplification method
methods genomic dna of cultured s. agalactiae was prepared and loop-mediated isothermal amplification (lamp) primers were designed based on the camp gene in bacteria. the optimum primer set was selected based on the reaction speed and specificity. the reaction result was monitored visually. the sensitivity and specificity of the lamp method were evaluated and compared with polymerase chain reac...
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Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae are the three main pathogens causing bovine mastitis, with great losses to the dairy industry. Rapid and specific loop-mediated isothermal amplification methods (LAMP) for identification and differentiation of these three pathogens are not available. With the 16S rRNA gene and 16S-23S rRNA intergenic spacers as target...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2013
ISSN: 0266-8254
DOI: 10.1111/lam.12114